Pre-eclampsia (PE) is a complication of pregnancy that affects 5–8% of women after 20 weeks of gestation. It is usually diagnosed based on the de novo onset of hypertension and proteinuria. Preexisting hypertension in women developing PE, also known as superimposed PE on chronic hypertension (SPE), leads to elevated risk of maternal and fetal mortality. PE is associated with an altered microRNA (miRNA) expression pattern in the placenta, suggesting that miRNA deregulation is involved in the pathogenesis of PE. Whether and how the miRNA expression pattern is changed in the SPE placenta remains unclear.
StatSoft_STATISTICA_10_Russian_Portable_10_.0.1011.0_x86_2011__RUS_OOXBD9.exe.torrent 热门搜索 GNE-203 张柏芝 逆流而上的你 上原瑞穗 蓝光 开苞 200GANA-1981 丑曦 PRED116 IPX-159. May 13, 2016 - Ion Torrent sequencing. Statistical analysis was performed using Statistica 10.0 (StatSoft, Inc., Tulsa, OK, USA). The collecting of tissue samples and small RNA isolation were supported by the Russian Federation President (grant no. Report of the National high blood pressure education program.
The present study analyzed the placental miRNA expression profile in pregnancies complicated by SPE. MiRNA expression profiles in SPE and normal placentas were investigated using an Ion Torrent sequencing system. Sequencing data were processed using a comprehensive analysis pipeline for deep miRNA sequencing (CAP-miRSeq). A total of 22 miRNAs were identified to be deregulated in placentas from patients with SPE. Horror tiles rpg maker vx ace cracked.
They included 16 miRNAs previously known to be associated with PE and 6 novel miRNAs. Among the 6 novel miRNAs, 4 were upregulated (miR-518a, miR-527, miR-518e and miR-4532) and 2 downregulated (miR-98 and miR-135b) in SPE placentas compared with controls. The present results suggest that SPE is associated with specific alterations in the placental miRNA expression pattern, which differ from alterations detected in PE placentas, and therefore, provide novel targets for further investigation of the molecular mechanisms underlying SPE pathogenesis. Introduction Pre-eclampsia (PE) is a complication of pregnancy characterized by the onset of hypertension and proteinuria after 20 weeks of gestation. It affects 5–8% of pregnant women and is one of the predominant causes of maternal and neonatal mortality and morbidity worldwide (,).
Women with chronic hypertension are 3–5 times more likely to develop PE than those with normal blood pressure (). Superimposed PE on chronic hypertension (SPE) is associated with poor pregnancy out comes (,). Despite the fact that the origin and pathogenesis of both PE and SPE have been extensively investigated (,,–), they remain unclear to date. Recent studies have reported the implication of microRNAs (miRNAs) in the development of PE (,). MiRNAs are a class of 21–25-nucleotide noncoding single-stranded small RNAs.
MiRNAs negatively regulate gene expression by binding to the 3′-untranslated region of the target mRNAs and thus may possess important control functions in diverse biological processes (,,). Aberrant expression of certain miRNAs has been identified in the placentas and blood from women with PE, suggesting that miRNA deregulation is involved in PE pathogenesis (,). Chang et al () reported that the top-ranked placental mRNA transcripts differed between patients with PE and those with SPE, suggesting that the pathogeneses of these two diseases are driven by different molecular mechanisms, which may include variations in the miRNA regulation network.